A man has been charged with one count of murder and two counts of attempted murder after allegedly stabbing three people.
Kane Kotwica, 39, of Longford in Gloucestershire, appeared at Cheltenham Magistrates' Court earlier where he was remanded into custody to next appear at Bristol Crown Court on 2 June.
Officers were called to a property in Bedford Street in Gloucester at about 07:40 BST on Wednesday following reports a number of people had been injured.
One of the three men taken to hospital for treatment died from his injuries at about 12:00. He has now been named by police as 53-year-old Matthew Cainey from Gloucester.
Gloucestershire Police said Mr Cainey's family were being supported by specially-trained officers.
One of the men who was injured has since been discharged but the other remains in hospital.
Bedford Street has now reopened to the public, however a scene guard remains in place at the address.